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KMID : 0613820060160061001
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Journal of Life Science
2006 Volume.16 No. 6 p.1001 ~ p.1005
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Identification of Rice Variety Using Simple Sequence Repeat (SSR) Marker
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Kwon Yong-Sham
Park Eun-Kyung
Park Chan-Ung
Bae Kyung-Mi
Lee Seung-In
Cho Il-Ho
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Abstract
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The objective of this study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for genetic diversity assessment and identification of rice varieties. The 23 primers selected from 50 SSR primers showed polymorphisms in the 21 rice varieties. The 2 to 9 SSR alleles were detected for each locus with an average of 3.00 alleles per locus. The polymorphism information content (PIC) ranged from 0.091 to 0.839. Based on band patterns, UPGMA cluster analysis was conducted. These varieties were separated into 4 groups corresponding to rice ecotype and pedigree information and genetic distance of cluster ranging from 0.59 to 0.92. The 4 SSR primer sets (RM206, RM225, RM418, RM478) selected from 23 polymorphic primers were differentiated all the rice variety from each other by markers genotypes. These markers may be used wide range of practical application in variety identification of rice.
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KEYWORD
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Genetic assessment, molecular marker, SSR, variety identification
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